High-performance Liquid Chromatography Separation of Glycerol Derivatives on a Chiral Stationary Phase
نویسندگان
چکیده
We report herein the separation of enantiomers of glycerol derivatives on a cellulose-modified chiral column (Chiracel OD) in normal-phase mode. This method is suitable for monitoring the progress of the reaction and for determination of the enantiomeric purity of products. The lipase Novozyme 435-catalysed enantioselective hydrolysis of 1-O-benzyl-2,3di-O-octanoyl-rac-glycerol has been shown to be an efficient method for preparation of 1-O-benzyl-2-O-octanoyl-sn-glycerol with high enantiomeric excess (ee > 95%).
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